Given that the 16S profiles from your gut look quite distinctive for intracellular and extracellular DNA [33], qPCR is expected to replicate the NGS-targeted Neighborhood framework equally quantitatively and qualitatively more carefully than circulation cytometry.

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Compared with movement cytometry that counts cells, qPCR and NGS both target bacterial DNA, like extracellular DNA derived from lysed germs. Extracellular DNA can be intrinsic or end result through the differential lysis of Gram-good and destructive micro organism throughout the frequent freeze-thawing before fecal DNA extraction.

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